Friday, February 22, 2013

Is Pertussis Evolving to Fight Vaccines?



Pertussis, more commonly known as whooping cough, has unfortunately shown a strong resurgence within the past few years not only in the United States, but in countries across the globe as well.  In fact, there were a reported 27, 550 cases of pertussis in the United States in 2010, marking the year as that with the most numerous cases of the infection since 1959.  This new-found prominence in the disease has in turn resulted in a variety of public service announcements, on the radio and television, warning the general public of the symptoms and the importance of vaccination, especially for those with infants, as they tend to be the group most susceptible to the infection.  Without a doubt, the most resonating part of the segments are the sound bites of infants first going into coughing fits before beginning to gasp for air, the characteristic whooping sound, placed intermittently throughout the duration of the message.  If the information in the public service announcement does not catch your attention, the pained sounds of sick infants definitely will.

Pertussis is a highly contagious respiratory disease caused by an infection by the bacteria Bordetella pertussis.  The bacteria attack the upper respiratory system, releasing toxins that cause inflammation.  This in turn causes those infected to descend into bursts of rapid coughs which are then followed by a period of attempting to draw in as much air as possible in order to recover from the depletion in oxygen levels in the body due to the long coughing spells.  The disease often leads to the development of pneumonia, and in the worst cases the depletion in oxygen levels can lead to brain damage and even death.  For this reason, physicians highly recommend vaccination to those in direct contact with infants as it has shown to significantly decrease the chance of spreading the disease.

Despite the effectiveness of vaccination, public health studies have shown that pertussis has again begun to reemerge, but unexpectedly in countries with highly vaccinated populations.  Though factors such as decreases in vaccine coverage and vaccine quality could play a role in this resurgence of the disease, researchers in the Netherlands hypothesize that the bacteria has evolved to resist the effects of vaccines.

To test this theory, the researchers collected bacteria strains from 1949 to 1996, grouping them into periods of 5 to 8 years, and determined the frequency of different DNA fingerprint types within each of said periods.  The results showed a distinct difference between in the fingerprints types found before and after usage of the vaccine became widespread.  Moreover, the results showed that genotypic diversity drastically decreased soon after implementation of the vaccine, suggesting that only those bacteria with the correct genetic coding were able to resist being wiped out.  However, over the years the genotypic diversity has increased, implying that those surviving strains have continued to adapt and mutate so as to remain unaffected by vaccines.

In addition, the researcher also investigated the effects of the polymorphism observed in pertussis toxin and pertactin, two important virulence factors necessary for the bacteria to be able to bind to the host’s cells.  Results showed the polymorphisms were non-conservative for the most part, which would imply that Darwinian selection plays an important role in this adaption found in the bacterial DNA.  Specifically, results showed that tandem repeats in the coding existed near the RGD amino acid motif, making the area quite susceptible to mutation as a result of slipped-strand mispairing during DNA replication.  Said mutations affect areas of the bacteria related to binding with T-cells, causing those bacteria with sequences most distinct from those found in the pre-vaccination era selected to be selected.  Because of the mutations, the receptor binding area has changed such that the host’s T-cells can no longer bind to the bacteria, meaning that the bacteria no longer has to fear being eliminated from the host.

Though the usage of vaccines to help fight the spread of pertussis has without a doubt been quite beneficial at reducing the number of pertussis-related deaths over the decades, it would seem that yet again the bacteria are adapting and evolving to resist the effects of said vaccines.  Those bacteria able to survive the initial wave of vaccines have evolved and given way to new mutants that are beginning to show resistance against T-cells.  Though this is most likely not the only reason why pertussis has shown an increase in activity within the past few years, it does open up more doors towards the continued effort to eventually bring immunity towards the disease hopefully sometime in the future.

Danielle Spencer

Word Count:  760

References:

1.  http://www.cdc.gov/pertussis/

2.  Mooi, Frits R., Inge H. M. van Loo, Audrey J. King. Adaptation of Bordetella pertussis to Vaccination: A Cause for Its Reemergence? Emerging Infectious Diseases. 7(3, Supplement): 526-528, 2001.

Image from: http://medblog.medlink-uk.net/gangnamlad/files/2013/02/whooping-cough.jpg




5 comments:

  1. I wonder why pertussis hasn't been completely eradicated like polio. Could it be because polio vaccination was mandatory? In the case of pertussis, it's voluntary, so some strains managed to survive the initial wave of vaccination and mutated to adapt to the new environment.

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  2. I think it is important to note that a new pertussis strain may have evolved to avoid the attacks of an immune system primed through vaccination to eliminate the old strain. A new vaccine can certainly be developed to train the immune system against the new strain of virus. Therefore this potential mutation is very similar to bacterial antibiotic resistance with the caveat that a new vaccine may be easier to produce and is much more disease specific than a new antibiotic.

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  3. Also notable is that sometimes when a disease does decrease so much in the population, as pertussis did after the initial vaccinations, then people no longer feel the need to be immunized. They don't see anyone around them getting sick from this disease and therefore do not feel a direct threat of illness. I don't know if there is any data showing a decrease in vaccination rates or simply that the evolution of the bacteria caused the new spikes in pertussis.
    Are there any plans to develop new pertussis vaccines targeting resistant strains of bacteria?

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  4. This is another great example of how our medical technology has been a wonderful factor in human survival, but has prevented our populations from directly evolving with the bacteria. As a result, we need to evolve our technologies, rather than ourselves, to keep up. Thankfully, this appears to be a bit easier!

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  5. I think this blog is interesting as I did a topic similar to this one! It makes you really think about the cost of medical vaccines when it comes to viruses because their rapid rate of development contributes to their ability to evolve so quickly.

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